Pyruvate Level
8 days
Pyruvate - lactate, spectrophotometry
Accredited Laboratory Partner
Contact Technical Support
Measures blood or urine pyruvic acid. Evaluates metabolic acidosis and mitochondrial disease.
Perchloric acid supernatant from oxalated Whole Blood
0.5 mL clear supernatant
Container: Gray-top (potassium oxalate/sodium fluoride) tube AND one LabCorp 8% perchloric acid tube
Patient should be in a fasting and resting state (should not exercise)
Draw blood into chilled gray-top tube. Immediately after blood is drawn, add 2 mLof whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). NOTE: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained. Shake mixture vigorously for about 30 seconds. Refrigerate for five minutes to ensure complete protein precipitation. Centrifuge 5 to 10 minutes at approximately 1500xg. Immediately transfer the CLEAR SUPERNATANT to a transport tube. Label the transport tube as "SUPERNATANT" and send to the laboratory. NOTE: A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.
| Temperature | Period |
|---|---|
| Room temperature | 5 days |
| Refrigerated | 7 days |
| Frozen | 7 days |
Pyruvate Level
8 days
Pyruvate - lactate, spectrophotometry
Accredited Laboratory Partner
Contact Technical Support
Measures blood or urine pyruvic acid. Evaluates metabolic acidosis and mitochondrial disease.
Perchloric acid supernatant from oxalated Whole Blood
0.5 mL clear supernatant
Container: Gray-top (potassium oxalate/sodium fluoride) tube AND one LabCorp 8% perchloric acid tube
Patient should be in a fasting and resting state (should not exercise)
Draw blood into chilled gray-top tube. Immediately after blood is drawn, add 2 mLof whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). NOTE: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained. Shake mixture vigorously for about 30 seconds. Refrigerate for five minutes to ensure complete protein precipitation. Centrifuge 5 to 10 minutes at approximately 1500xg. Immediately transfer the CLEAR SUPERNATANT to a transport tube. Label the transport tube as "SUPERNATANT" and send to the laboratory. NOTE: A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.
| Temperature | Period |
|---|---|
| Room temperature | 5 days |
| Refrigerated | 7 days |
| Frozen | 7 days |