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Methotrexate Level

Order Code
11251021
CPT
80204
Test Details
Synonyms

MTX

TAT

Up to 24 hours

Methodology

Homogenous Enzyme Immunoassay

Performing Location

Accredited NRL Laboratory

Testing Frequency

Daily

Test Overview

Methotrexate is a type of medication called an anti-metabolite, specifically antifolate which inhibits the enzyme Difydrofolate Reductase, which is important for DNA sysnthesis.
It acts as a treatment for rheumatoid arthritis by decreasing immune system activity, for psoriasis by slowing the growth of skin cells and treats cancer by slowing the growth of cancer cells.
Methotrexate is measured to ensure the medication is being cleared appropriately and verifies that a nontoxic concentration has been attained following therapy.

Specimen Type

Plasma or Serum

Volume

0.5 mL

Specimen Container

Serum Separator Tube (SST; Gold Top) Plain Serum Tube (Red Top) Lithium Heparin Plasma (Green Top) EDTA Plasma (Lavender Top)

Patient Preparation

Seek guidance from your physician prior to sample collection.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated 14 days at 2 to 8° C
Frozen 26 weeks at -20° C
Order Code
11251021
CPT
80204
Test Details
Synonyms

MTX

TAT

Up to 24 hours

Methodology

Homogenous Enzyme Immunoassay

Performing Location

Accredited NRL Laboratory

Testing Frequency

Daily

Test Overview

Methotrexate is a type of medication called an anti-metabolite, specifically antifolate which inhibits the enzyme Difydrofolate Reductase, which is important for DNA sysnthesis.
It acts as a treatment for rheumatoid arthritis by decreasing immune system activity, for psoriasis by slowing the growth of skin cells and treats cancer by slowing the growth of cancer cells.
Methotrexate is measured to ensure the medication is being cleared appropriately and verifies that a nontoxic concentration has been attained following therapy.

Specimen Type

Plasma or Serum

Volume

0.5 mL

Specimen Container

Serum Separator Tube (SST; Gold Top) Plain Serum Tube (Red Top) Lithium Heparin Plasma (Green Top) EDTA Plasma (Lavender Top)

Patient Preparation

Seek guidance from your physician prior to sample collection.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated 14 days at 2 to 8° C
Frozen 26 weeks at -20° C