Lipid Cascade With Reflex to Lipoprotein Particle Assessment by NMR (With Graph if Reflexed)

5 days

Lipid profile: Enzymatic, lipoprotein reflex: Nuclear magnetic resonance (NMR)

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Starts with standard lipid panel and reflexes to detailed lipoprotein particle analysis by NMR.

Lipid profile: Serum (preferred) or Plasma Lipoprotein reflex: Spun NMR LipoTube (preferred) Serum from a plain red-top tube Plasma from a lavender-top (EDTA-no gel) tube or green-top (heparin-no gel) tube

2 mL each tube

Lipid profile: Gel-barrier transport, green-top (heparin), or lavender-top (EDTA) tube. Lipoprotein reflex: One of the following: NMR LipoTube (black-and-yellow-top tube), preferred, plain red-top tube, lavender-top (EDTA-no gel) tube, or green-top (heparin-no gel) tube. Two specimens must be submitted with this profile, one for the lipid profile and another for lipoprotein reflex testing.

Patient should be fasting for a minimum of eight to 12 hours

Lipid profile: Separate serum or plasma from cells within 45 minutes of collection.

Lipoprotein reflex: Collect specimen in NMR LipoTube (black-and-yellow-top gel-barrier tube), which is the preferred tube. Keep upright at room temperature for 30 minutes and allow to clot. Immediately after clotting, centrifuge at 1600 to 1800 xg for 10 to 15 minutes. The NMR LipoTube should then be stored at 2°C to 8°C until shipped. Do not open NMR LipoTube.

For plain serum (red-top, no gel) keep upright at room temperature for 45 minutes and allow to clot. Centrifuge immediately after clotting. Separate serum and transfer to a plastic transport tube. The serum should then be stored at 2°C to 8°C until shipped.

For EDTA plasma (lavender-top, no gel), invert tube several times to mix contents and centrifuge immediately after collection. Separate plasma and transfer to a plastic transport tube. The plasma should then be stored at 2°C to 8°C until shipped.

Serum or plasma drawn in gel-barrier collection tubes other than the NMR LipoTube should not be used.