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Alpha-1-Antitrypsin Deficiency DNA Analysis

Order Code
511881
CPT
81332
Test Details
Synonyms

A1AT

TAT

16 days

Methodology

Two variants are analyzed: c.1096 G>A (p. Glu366Lys), commonly referred to as the Z allele or PI*Z c.863 A>T (p.Glu288Val), commonly referred to as the S allele or PI*S. DNA analysis of the S and Z alleles in the SERPINA1 gene (NM_000295.4) is performed by multiplex allele-specific PCR amplification followed by gel electrophoresis.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Genetic test for mutations in the SERPINA1 gene. Confirms diagnosis of alpha-1-antitrypsin deficiency.

Specimen Type

Whole Blood amniotic fluid chorionic villus sample (CVS) (submission of maternal Blood is required for fetal testing) or LabCorp buccal swab kit (buccal swab collection kit contains instructions for use of a buccal swab)

Volume

7 mL whole blood, 10 mL amniotic fluid, 20 mg CVS OR LabCorp buccal swab kit

Specimen Container

Lavender-top (EDTA) tube or yellow-top (ACD) tube, sterile plastic conical tube OR two confluent T-25 flasks for fetal testing, OR LabCorp buccal swab kit

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature Contact Technical Support
Refrigerated Contact Technical Support
Order Code
511881
CPT
81332
Test Details
Synonyms

A1AT

TAT

16 days

Methodology

Two variants are analyzed: c.1096 G>A (p. Glu366Lys), commonly referred to as the Z allele or PI*Z c.863 A>T (p.Glu288Val), commonly referred to as the S allele or PI*S. DNA analysis of the S and Z alleles in the SERPINA1 gene (NM_000295.4) is performed by multiplex allele-specific PCR amplification followed by gel electrophoresis.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Genetic test for mutations in the SERPINA1 gene. Confirms diagnosis of alpha-1-antitrypsin deficiency.

Specimen Type

Whole Blood amniotic fluid chorionic villus sample (CVS) (submission of maternal Blood is required for fetal testing) or LabCorp buccal swab kit (buccal swab collection kit contains instructions for use of a buccal swab)

Volume

7 mL whole blood, 10 mL amniotic fluid, 20 mg CVS OR LabCorp buccal swab kit

Specimen Container

Lavender-top (EDTA) tube or yellow-top (ACD) tube, sterile plastic conical tube OR two confluent T-25 flasks for fetal testing, OR LabCorp buccal swab kit

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature Contact Technical Support
Refrigerated Contact Technical Support