ADAMTS13 Activity

von Willebrand Factor-cleaving Protease

6 days

Liquid chromatography‐tandem mass spectrometry (LC/MS-MS) assay

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Assesses the activity of ADAMTS13 enzyme. Used in diagnosing thrombotic thrombocytopenic purpura (TTP).

Plasma

0.5 mL

Blue top (sodium citrate) tube

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Serum gel-barrier tubes and serum tubes with clot initiators should aslo be collected after the citrate tubes. Centrifuge and carefully remove the plasma using a plastic transfer pipette, being careful not to disturb the cells.