Pregnenolone, 17-Hydroxy (Two Specimens)
16 days
High-pressure liquid chromatography (HPLC) tandem mass spectrometry
Transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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Measures 17-hydroxypregnenolone levels before and after ACTH stimulation to assess adrenal steroidogenesis. Helps diagnose congenital adrenal hyperplasia and enzyme deficiencies like 3-HSD deficiency
Serum (preferred) or Plasma
1 mL (each specimen),
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube
No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.
Draw blood for baseline 17-OH pregnenolone. Inject cosyntropin 250 ug IM or IV (If IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over 2 minutes). Draw blood for stimulated 17-OH pregnenolone 60 minutes after.
| Temperature | Period |
|---|---|
| Frozen | 2 years |