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von Willebrand Factor Multimers

Order Code
117245
CPT
To be determined. Updates will be made when available.
Test Details
Synonyms

vWF Multimer Analysis

TAT

11 days

Methodology

The Sebia Hydragel™ assay employs an agarose gel for the separation of plasma proteins according to their molecular weight. The electrophoretic separation is carried out after sample treatment with an anionic detergent. This treatment disrupts the three-d

Remarks

Causes for Rejection
Lipemia; icteric specimen; hemolysis; clotted specimen; specimen contaminated with heparin (i.e., drawn with blood gases)

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

This test is used to measure the relative levels of von Willebrand low molecular weight multimers (LMWM), intermediate molecular weight multimers (IMWM) and high molecular weight multimers (HMWM) in plasma.

Test Includes:
This test includes von Willebrand factor activity (VWF:Ag); von Willebrand factor antigen (VWF:Ac); VWF:Ag/ VWF:Ac ratio; and von Willebrand Multimer results including percent Intermediate and High Molecular Weight Fractions.

Specimen Type

Plasma

Volume

2 mL

Specimen Container

Blue top (sodium citrate) tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.24 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.25,26 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples unless the sample is collected using a winged (butterfly) collection system. With a winged blood collection set, a discard tube should be drawn first to account for the dead space of the tubing and prevent under-filling of the evacuated tube.27,28 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternative anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes.

Specimen Stability
Temperature Period
Frozen Contact Technical Support
Order Code
117245
CPT
To be determined. Updates will be made when available.
Test Details
Synonyms

vWF Multimer Analysis

TAT

11 days

Methodology

The Sebia Hydragel™ assay employs an agarose gel for the separation of plasma proteins according to their molecular weight. The electrophoretic separation is carried out after sample treatment with an anionic detergent. This treatment disrupts the three-d

Remarks

Causes for Rejection
Lipemia; icteric specimen; hemolysis; clotted specimen; specimen contaminated with heparin (i.e., drawn with blood gases)

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

This test is used to measure the relative levels of von Willebrand low molecular weight multimers (LMWM), intermediate molecular weight multimers (IMWM) and high molecular weight multimers (HMWM) in plasma.

Test Includes:
This test includes von Willebrand factor activity (VWF:Ag); von Willebrand factor antigen (VWF:Ac); VWF:Ag/ VWF:Ac ratio; and von Willebrand Multimer results including percent Intermediate and High Molecular Weight Fractions.

Specimen Type

Plasma

Volume

2 mL

Specimen Container

Blue top (sodium citrate) tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.24 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.25,26 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples unless the sample is collected using a winged (butterfly) collection system. With a winged blood collection set, a discard tube should be drawn first to account for the dead space of the tubing and prevent under-filling of the evacuated tube.27,28 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternative anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes.

Specimen Stability
Temperature Period
Frozen Contact Technical Support