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Sickle Cell Fetal Analysis

Order Code
482091
CPT
81361
Test Details
Synonyms

Sickle cell disease; hemoglobin SC disease

TAT

18 days (If culture is needed, an additional 14 - 21 days may be required. Additional culture fee may be applied.)

Methodology

Sickle cell analysis: HbS (c.20A>T, p.Glu7Val) and HbC (c.19G>A, p.Glu7Lys) pathogenic variants are identified by Sanger sequencing, capillary gel electrophoresis and fluorescence detection. Maternal cell contamination analysis (MCC): Analysis of short tandem repeat markers by multiplex fluorescent polymerase chain reaction (PCR) and capillary electrophoresis.

Remarks

This test is for fetal testing only. It is not intended for routine sickle cell screening. LabCorp clients with 8-digit client account numbers should call 800-345-4363 and LabCorp Genetics & Women's Health clients with 6-digit client/subclient account numbers should call 800-255-7357 to speak with a laboratory genetic coordinator before collecting specimens. In some circumstances, specimens from both parents and other family members may be required. All fetal specimens, including cord blood, must be accompanied by a maternal blood, PurFlock buccal swab kit or Oragene Dx 500 saliva kit for maternal cell contamination (MCC). A separate requisition should be submitted with the maternal specimen.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Tests fetal DNA for sickle cell trait or disease.

Specimen Type

Amniotic fluid or chorionic villus sample (CVS) or cultured cells or cord Blood (Direct amniotic fluid or CVS specimen may be submitted additional culture fee may be applied)

Volume

Amniotic fluid: 10 mL, CVS: 10 mg, amniotic fluid and CVS culture: one confluent T-25 flask or 4 mL cord blood (If amniotic fluid or CVS are cultured at another facility, please maintain back-up cultures.)

Specimen Container

Amniotic fluid or CVS: sterile plastic conical tube or T-25 flask, cord blood: yellow-top (ACD-A) or lavender-top (EDTA) tubes

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature Contact Technical Support
Order Code
482091
CPT
81361
Test Details
Synonyms

Sickle cell disease; hemoglobin SC disease

TAT

18 days (If culture is needed, an additional 14 - 21 days may be required. Additional culture fee may be applied.)

Methodology

Sickle cell analysis: HbS (c.20A>T, p.Glu7Val) and HbC (c.19G>A, p.Glu7Lys) pathogenic variants are identified by Sanger sequencing, capillary gel electrophoresis and fluorescence detection. Maternal cell contamination analysis (MCC): Analysis of short tandem repeat markers by multiplex fluorescent polymerase chain reaction (PCR) and capillary electrophoresis.

Remarks

This test is for fetal testing only. It is not intended for routine sickle cell screening. LabCorp clients with 8-digit client account numbers should call 800-345-4363 and LabCorp Genetics & Women's Health clients with 6-digit client/subclient account numbers should call 800-255-7357 to speak with a laboratory genetic coordinator before collecting specimens. In some circumstances, specimens from both parents and other family members may be required. All fetal specimens, including cord blood, must be accompanied by a maternal blood, PurFlock buccal swab kit or Oragene Dx 500 saliva kit for maternal cell contamination (MCC). A separate requisition should be submitted with the maternal specimen.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Tests fetal DNA for sickle cell trait or disease.

Specimen Type

Amniotic fluid or chorionic villus sample (CVS) or cultured cells or cord Blood (Direct amniotic fluid or CVS specimen may be submitted additional culture fee may be applied)

Volume

Amniotic fluid: 10 mL, CVS: 10 mg, amniotic fluid and CVS culture: one confluent T-25 flask or 4 mL cord blood (If amniotic fluid or CVS are cultured at another facility, please maintain back-up cultures.)

Specimen Container

Amniotic fluid or CVS: sterile plastic conical tube or T-25 flask, cord blood: yellow-top (ACD-A) or lavender-top (EDTA) tubes

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature Contact Technical Support