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Peri-nuclear pattern ANCA

Order Code
11250015
CPT
86036, 86037
Test Details
Synonyms

P-ANCA, MPO Antibodies

TAT

5 days

Methodology

Indirect Immunofluorescence Assay

Performing Location

Accredited NRL Laboratory

Testing Frequency

Twice per week (Monday and Thursday)

Test Overview

Antibodies against myeloperoxidase (MPO) are referred to as p-ANCA subtype, since they form a perinuclear fluorescence pattern. This ANCA-fluorescent (antineutrophil cytoplasmic antibodies) subtype includes other antibodies such as antibodies against lactoferrin, cathepsin G or elastase. However, in at least 60% of p-ANCA reactivity cases, the main antigen is MPO. Anti-MPO antibodies are primarily considered an important indicator for progressing nephritis; they are largely present in patients with severe renal impairment. They are also important for diagnosing Churg-Strauss syndrome and microscopic polyangiitis. The presence or absence of antibodies against MPO and PR3 in combination with the positivity of antinuclear antibodies may be regarded as a differentiating marker between ANCA-associated vasculitis and SLE-induced vasculitis.

Specimen Type

Serum

Volume

1 mL

Specimen Container

Gel barrier tube (preferred) or red top tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated 2 days
Frozen 7 days
Order Code
11250015
CPT
86036, 86037
Test Details
Synonyms

P-ANCA, MPO Antibodies

TAT

5 days

Methodology

Indirect Immunofluorescence Assay

Performing Location

Accredited NRL Laboratory

Testing Frequency

Twice per week (Monday and Thursday)

Test Overview

Antibodies against myeloperoxidase (MPO) are referred to as p-ANCA subtype, since they form a perinuclear fluorescence pattern. This ANCA-fluorescent (antineutrophil cytoplasmic antibodies) subtype includes other antibodies such as antibodies against lactoferrin, cathepsin G or elastase. However, in at least 60% of p-ANCA reactivity cases, the main antigen is MPO. Anti-MPO antibodies are primarily considered an important indicator for progressing nephritis; they are largely present in patients with severe renal impairment. They are also important for diagnosing Churg-Strauss syndrome and microscopic polyangiitis. The presence or absence of antibodies against MPO and PR3 in combination with the positivity of antinuclear antibodies may be regarded as a differentiating marker between ANCA-associated vasculitis and SLE-induced vasculitis.

Specimen Type

Serum

Volume

1 mL

Specimen Container

Gel barrier tube (preferred) or red top tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated 2 days
Frozen 7 days