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IgVH Mutation Analysis

Order Code
113753
CPT
81263
Test Details
Synonyms

Immunoglobulin Heavy Chain Variable Region Mutation Analysis

TAT

12 days

Methodology

The immunoglobulin heavy-chain gene variable region (IgVH) somatic hypermutation analysis is performed using extracted patient RNA as starting material. Subsequent amplification of the IgH gene is performed by polymerase chain reaction (PCR). The PCR products are isolated and sequenced. The nucleotide sequence is compared to a consensus germLine sequence data base for that VH gene family.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Evaluates somatic hypermutation status of the immunoglobulin heavy chain variable region gene (IgVH). Prognostic marker in chronic lymphocytic leukemia (CLL), where mutated status is associated with better outcomes.

Specimen Type

Peripheral Blood or Bone Marrow

Volume

1 mL

Specimen Container

Lavender-top (EDTA) tube, green-top (heparin) tube or yellow-top (ACD) tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature 5 days
Order Code
113753
CPT
81263
Test Details
Synonyms

Immunoglobulin Heavy Chain Variable Region Mutation Analysis

TAT

12 days

Methodology

The immunoglobulin heavy-chain gene variable region (IgVH) somatic hypermutation analysis is performed using extracted patient RNA as starting material. Subsequent amplification of the IgH gene is performed by polymerase chain reaction (PCR). The PCR products are isolated and sequenced. The nucleotide sequence is compared to a consensus germLine sequence data base for that VH gene family.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Evaluates somatic hypermutation status of the immunoglobulin heavy chain variable region gene (IgVH). Prognostic marker in chronic lymphocytic leukemia (CLL), where mutated status is associated with better outcomes.

Specimen Type

Peripheral Blood or Bone Marrow

Volume

1 mL

Specimen Container

Lavender-top (EDTA) tube, green-top (heparin) tube or yellow-top (ACD) tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature 5 days