Bethesda Titer
9 days
The factor X inhibitor (Bethesda titer) assay is performed using a prothrombin time-based system, using thromboplastin reagent.6 Serial dilutions are made of patient plasma with veronal buffered saline, then mixed with normal plasma containing close to 100% factor X activity and are then incubated for two hours. A PT-based factor X assay using factor X-depleted plasma substrate is then performed on these incubated mixtures. Results are compared to those of incubated normal plasma. One Bethesda unit is defined as the amount of factor X inhibitor that neutralized 0.5 IU of factor X in this system. The number of serial dilutions tested is based on the anticipated level of the inhibitor.
Test Includes: Activated partial thromboplastin time (aPTT), aPTT 1:1 mix with normal plasma, aPTT 1:1 mix with saline, factor X activity, factor X Bethesda titer
Accredited Laboratory Partner
Contact Technical Support
Assesses presence of inhibitors against Factor X. Used in acquired bleeding disorders or factor replacement resistance.
Plasma
6 mL (2 mL in each of three tubes)
Blue top (sodium citrate) tube
No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.
Citrated Plasma samples should be collected by double centrifugation
| Temperature | Period |
|---|---|
| Frozen | checking |
Bethesda Titer
9 days
The factor X inhibitor (Bethesda titer) assay is performed using a prothrombin time-based system, using thromboplastin reagent.6 Serial dilutions are made of patient plasma with veronal buffered saline, then mixed with normal plasma containing close to 100% factor X activity and are then incubated for two hours. A PT-based factor X assay using factor X-depleted plasma substrate is then performed on these incubated mixtures. Results are compared to those of incubated normal plasma. One Bethesda unit is defined as the amount of factor X inhibitor that neutralized 0.5 IU of factor X in this system. The number of serial dilutions tested is based on the anticipated level of the inhibitor.
Test Includes: Activated partial thromboplastin time (aPTT), aPTT 1:1 mix with normal plasma, aPTT 1:1 mix with saline, factor X activity, factor X Bethesda titer
Accredited Laboratory Partner
Contact Technical Support
Assesses presence of inhibitors against Factor X. Used in acquired bleeding disorders or factor replacement resistance.
Plasma
6 mL (2 mL in each of three tubes)
Blue top (sodium citrate) tube
No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.
Citrated Plasma samples should be collected by double centrifugation
| Temperature | Period |
|---|---|
| Frozen | checking |