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EWSR1 Oncology FISH

Order Code
510379
CPT
88271x2, 88275
Test Details
Synonyms

Clear Cell Sarcoma Gene, Peripheral Neuroectodermal Tumor Gene, Desmoplastic Small Round Cell Tumor Gene, Ewing Sarcoma Breakpoint Region 1

TAT

12 days

Methodology

Fluorescence in situ hybridization (FISH)

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Detects EWSR1 gene rearrangements. Used in sarcoma diagnosis.

Specimen Type

Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei, OR bone marrow touch prep slides. Whole blood, bone marrow, CSF, FNA, paraffin block are also acceptable with additional charge

Volume

5mL whole blood (pediatric), 3mL bone marrow, fixed-cellpellet from a cytogenetic analysis, 2 slides with metaphaseand/or interphase nuclei, 2 touch preparation slides OR 3 slides from paraffin block.

Specimen Container

Green-top (sodium heparin) tube; pediatric Vacutainer(R) is optimal. OR lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature Contact Technical Support
Order Code
510379
CPT
88271x2, 88275
Test Details
Synonyms

Clear Cell Sarcoma Gene, Peripheral Neuroectodermal Tumor Gene, Desmoplastic Small Round Cell Tumor Gene, Ewing Sarcoma Breakpoint Region 1

TAT

12 days

Methodology

Fluorescence in situ hybridization (FISH)

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Detects EWSR1 gene rearrangements. Used in sarcoma diagnosis.

Specimen Type

Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei, OR bone marrow touch prep slides. Whole blood, bone marrow, CSF, FNA, paraffin block are also acceptable with additional charge

Volume

5mL whole blood (pediatric), 3mL bone marrow, fixed-cellpellet from a cytogenetic analysis, 2 slides with metaphaseand/or interphase nuclei, 2 touch preparation slides OR 3 slides from paraffin block.

Specimen Container

Green-top (sodium heparin) tube; pediatric Vacutainer(R) is optimal. OR lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature Contact Technical Support