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Cytoplasmic pattern ANCA

Order Code
11250014
CPT
86036, 86037
Test Details
Synonyms

C-ANCA, Proteinase 3 Antibodies

TAT

5 days

Methodology

Indirect Immunofluorescence Assay

Performing Location

Accredited NRL Laboratory

Testing Frequency

Twice per week (Monday and Thursday)

Test Overview

The most common target antigens of ANCA-associated vasculitis are proteinase 3 or myeloperoxidase. Antibodies against proteinase 3 (PR3) are referred to as c-ANCA fluorescent subtype, namely cytoplasmic antibodies (granular cytoplasmic fluorescence). PR3 is a neutral serine proteinase 3, also known as Wegener's autoantigen. Antibodies against PR3 are a highly specific marker in diagnosing Wegener's granulomatosis.

Specimen Type

Serum

Volume

1 mL

Specimen Container

Gel barrier tube (preferred) or red top tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated 2 days
Frozen 7 days
Order Code
11250014
CPT
86036, 86037
Test Details
Synonyms

C-ANCA, Proteinase 3 Antibodies

TAT

5 days

Methodology

Indirect Immunofluorescence Assay

Performing Location

Accredited NRL Laboratory

Testing Frequency

Twice per week (Monday and Thursday)

Test Overview

The most common target antigens of ANCA-associated vasculitis are proteinase 3 or myeloperoxidase. Antibodies against proteinase 3 (PR3) are referred to as c-ANCA fluorescent subtype, namely cytoplasmic antibodies (granular cytoplasmic fluorescence). PR3 is a neutral serine proteinase 3, also known as Wegener's autoantigen. Antibodies against PR3 are a highly specific marker in diagnosing Wegener's granulomatosis.

Specimen Type

Serum

Volume

1 mL

Specimen Container

Gel barrier tube (preferred) or red top tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated 2 days
Frozen 7 days