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Cortisol ACTH Stimulation

Order Code
140761
CPT
82533x2
Test Details
Synonyms

Addison's Disease Test, Cosyntropin Stimulation

TAT

6 days

Methodology

Electrochemiluminescence immunoassay (ECLIA)

Remarks

Draw blood for baseline cortisol. Inject cosyntropin 250 μg IM or IV (if IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated cortisol 60 minutes after. Treatment for patients in suspected adrenal crisis should not be delayed while waiting for the results of ACTH (cosyntropin) stimulation testing. Formal confirmation of diagnosis can be carried out after clinical recovery.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Single or two-sample ACTH stimulation test.

Specimen Type

Serum

Volume

1 mL (each specimen)

Specimen Container

Red-top tube or gel-barrier tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

If tube other than gel-barrier tube is used, transfer separated Serum to a plastic transport tube. NOTE: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same request form

Specimen Stability
Temperature Period
Room temperature 14 days
Refrigerated 14 days
Frozen 14 days
Freeze/thaw cycles Stable x3
Order Code
140761
CPT
82533x2
Test Details
Synonyms

Addison's Disease Test, Cosyntropin Stimulation

TAT

6 days

Methodology

Electrochemiluminescence immunoassay (ECLIA)

Remarks

Draw blood for baseline cortisol. Inject cosyntropin 250 μg IM or IV (if IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated cortisol 60 minutes after. Treatment for patients in suspected adrenal crisis should not be delayed while waiting for the results of ACTH (cosyntropin) stimulation testing. Formal confirmation of diagnosis can be carried out after clinical recovery.

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Single or two-sample ACTH stimulation test.

Specimen Type

Serum

Volume

1 mL (each specimen)

Specimen Container

Red-top tube or gel-barrier tube

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

If tube other than gel-barrier tube is used, transfer separated Serum to a plastic transport tube. NOTE: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same request form

Specimen Stability
Temperature Period
Room temperature 14 days
Refrigerated 14 days
Frozen 14 days
Freeze/thaw cycles Stable x3