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BRAF Somatic Mutation Tumor

Order Code
1723123
CPT
81210, 88381
Test Details
Synonyms

Melanoma, lung, and thyroid cancer, among others. Mutations in V600 mutation codon is of diagnostic importance in hairy cell leukemia, Langerhans cell histiocytosis, and Erdheim-Chester Disease

TAT

72 hrs

Methodology

QPCR

Remarks

Qualitative detection of V600E/E2/D and V600K/R/M mutations in codon 600 of the BRAF gene

Performing Location

Accredited NRL Laboratory

Testing Frequency

Contact Technical Support

Test Overview

Detect somatic BRAF mutations in tumors; personalized cancer treatment.

Specimen Type

FFPE, gDNA; Bone Marrow

Volume

Formalin-Fixed Paraffin-Embedded tissue block (preferred). 2 scrolls or 5 unstained slides in a sterile container (5 µm sections), along with an H&E-Stained Slide for tumor cell content assessment.DNA extracted from other sample types can also be tested (total DNA should be >50ng with at least 2-5µl provided)

Specimen Container

sterile container; EDTA for Bone marrow

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature FFPE & Slides
Refrigerated gDNA: 30 days if Refrigerated or 2 years if Frozen; Bone Marrow: 7 days
Order Code
1723123
CPT
81210, 88381
Test Details
Synonyms

Melanoma, lung, and thyroid cancer, among others. Mutations in V600 mutation codon is of diagnostic importance in hairy cell leukemia, Langerhans cell histiocytosis, and Erdheim-Chester Disease

TAT

72 hrs

Methodology

QPCR

Remarks

Qualitative detection of V600E/E2/D and V600K/R/M mutations in codon 600 of the BRAF gene

Performing Location

Accredited NRL Laboratory

Testing Frequency

Contact Technical Support

Test Overview

Detect somatic BRAF mutations in tumors; personalized cancer treatment.

Specimen Type

FFPE, gDNA; Bone Marrow

Volume

Formalin-Fixed Paraffin-Embedded tissue block (preferred). 2 scrolls or 5 unstained slides in a sterile container (5 µm sections), along with an H&E-Stained Slide for tumor cell content assessment.DNA extracted from other sample types can also be tested (total DNA should be >50ng with at least 2-5µl provided)

Specimen Container

sterile container; EDTA for Bone marrow

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Room temperature FFPE & Slides
Refrigerated gDNA: 30 days if Refrigerated or 2 years if Frozen; Bone Marrow: 7 days