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Acid-fast (Mycobacteria) Smear and Culture with Reflex to Identification and Susceptibility Testing

Order Code
183764
CPT
87206, 87116
Test Details
Synonyms

AFB Smear and Culture with Reflex to ID and DST

TAT

60 days

Methodology

Concentrated smears are stained with auramine/rhodamine and read by fluorescence microscopy, broth-based and/or agar-based culture. Culture is held for six weeks before negative is reported. Organisms from culture are identified by use of real-time polymerase chain reaction (PCR) and/or MALDI-TOF and/or nucleic acid sequencing. Susceptibility tests are done by broth-based and conventional methods.

Remarks

If reflex testing is performed, concomitant CPT codes/charges will apply

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Detects mycobacteria including tuberculosis. Positive cultures are further tested to identify species and antibiotic susceptibility.

Specimen Type

First morning sputum (not saliva) (three separate specimens from three separate days are recommended) fasting gastric aspirate induced sputum Whole Blood tissue biopsy bronchial aspirate Urine skin CSF Bone Marrow body fluid stool Swabs of exudate from skin sources are acceptable otherwise swab specimens should not be submitted Collect aspirate using sterile nonbacteriostatic saline or other noninhibitory medium Do not send syringe it will be rejected

Volume

5-10 mL sputum, bronchoalveolar lavage (BAL) or other respiratory specimen, 10 mL whole blood, 2 cm tissue, 5 mL CSF, 5-10 mL bone marrow (or as much as possible), 5-10 mL body fluid (pleural, pericardial, chronic peritoneal dialysate), 5-10 mL gastric aspirate/lavage (neutralized to pH 7 with sodium carbonate within 4 hours of collection), 20-40 mL urine (first morning specimen), 5 mL or 1 g stool, biopsy of skin

Specimen Container

Sterile container with tight screw-cap seal or green-top (sodium heparin) tube or yellow-top (SPS) tube or Para-Pak® White

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated Contact Technical Support
Order Code
183764
CPT
87206, 87116
Test Details
Synonyms

AFB Smear and Culture with Reflex to ID and DST

TAT

60 days

Methodology

Concentrated smears are stained with auramine/rhodamine and read by fluorescence microscopy, broth-based and/or agar-based culture. Culture is held for six weeks before negative is reported. Organisms from culture are identified by use of real-time polymerase chain reaction (PCR) and/or MALDI-TOF and/or nucleic acid sequencing. Susceptibility tests are done by broth-based and conventional methods.

Remarks

If reflex testing is performed, concomitant CPT codes/charges will apply

Performing Location

Accredited Laboratory Partner

Testing Frequency

Contact Technical Support

Test Overview

Detects mycobacteria including tuberculosis. Positive cultures are further tested to identify species and antibiotic susceptibility.

Specimen Type

First morning sputum (not saliva) (three separate specimens from three separate days are recommended) fasting gastric aspirate induced sputum Whole Blood tissue biopsy bronchial aspirate Urine skin CSF Bone Marrow body fluid stool Swabs of exudate from skin sources are acceptable otherwise swab specimens should not be submitted Collect aspirate using sterile nonbacteriostatic saline or other noninhibitory medium Do not send syringe it will be rejected

Volume

5-10 mL sputum, bronchoalveolar lavage (BAL) or other respiratory specimen, 10 mL whole blood, 2 cm tissue, 5 mL CSF, 5-10 mL bone marrow (or as much as possible), 5-10 mL body fluid (pleural, pericardial, chronic peritoneal dialysate), 5-10 mL gastric aspirate/lavage (neutralized to pH 7 with sodium carbonate within 4 hours of collection), 20-40 mL urine (first morning specimen), 5 mL or 1 g stool, biopsy of skin

Specimen Container

Sterile container with tight screw-cap seal or green-top (sodium heparin) tube or yellow-top (SPS) tube or Para-Pak® White

Patient Preparation

No special preparation is required for this test. You may eat, drink, and take your medications as normal, unless instructed otherwise by your healthcare provider.

Collection

This test does not require any special collection or pre-analytical handling. Follow routine specimen collection protocols.

Specimen Stability
Temperature Period
Refrigerated Contact Technical Support