The Center of Excellence for Tuberculosis Testing implements all quality performance standards required to safely and effectively culture Mycobacterium tuberculosis, the causative agent for Tuberculosis (TB). To perform such testing, NRL has ensured that its laboratory, with intricate design, containment, and equipment capabilities, comply with requirements for a Biosafety Level 3 (BSL-3) laboratory.  This set up will enable NRL to also test for other diseases of global concern, such as SARS Coronavirus, Brucella, and Yellow fever. 

The TB laboratory is located at our main testing site at ICAD, Abu Dhabi, within the frames of the Microbiology laboratory.

NRL Test Code

Order Code Name

Specimen type

Volume

Specimen container

Storage

Specimen Stability

TAT*days

Testing Frequency

Methodology

9924055

 Acid Fast (Mycobacteria) Smear

Any clinical material

The following are examples:

  • First morning sputum (not saliva),  fasting gastric aspirate, induced sputum, tissue, biopsy, bronchial aspirate (washings/lavage), urine, skin, cerebrospinal fluid (CSF), body fluid, stool.  Swabs of exudate from skin sources are acceptable, otherwise swab specimens should not be submitted. 

Note: Please call Micro lab for blood or bone marrow samples

5 mL sputum or respiratory aspirate, 2 cm³ tissue, 5 mL CSF, 50 mL body fluid (eg, gastric aspirate/lavage, urine, pleural, pericardial, chronic peritoneal dialysate), 10 mL stool, biopsy of skin

Sterile container

Refrigerate

72 hours at 2-8 °C

1 day

Daily

Concentrated smears are stained with auramine and read by fluorescence microscopy

9924063

Acid Fast (Mycobacteria) Smear and Culture with Reflex to ID and Susceptibility

Sterile container

Refrigerate

72 hours at 2-8 °C

42 days

Daily

MGIT™ 960 mycobacterial detection system

9924061

Acid Fast (Mycobacteria) Smear and Culture with Reflex to ID

Sterile container

Refrigerate

72 hours at 2-8 °C

42 days

Daily

MGIT™ 960 mycobacterial detection system

9924057

Acid Fast (Mycobacteria) Culture and Identification

Sterile container

Refrigerate

72 hours at 2-8 °C

42 days

Daily

MGIT™ 960 mycobacterial detection system

9924059

Acid Fast (Mycobacteria) Antibiotic Susceptibilities

Pure culture isolate of Mycobacterium tuberculosis. 

1 culture medium

Growth should be from a positive MGIT™ tube (i.e. instrument flagged “positive” MGIT™ tube confirmed as positive for the presence of acid-fast bacilli), a non-selective solid agar medium, or an egg-based medium (i.e. Middlebrook 7H11, Lowenstein-Jensen, etc).

Culture medium must be  securely sealed

Ambient

NA

13-21 days

Daily

MGIT™ 960 mycobacterial detection system

 

Clinical significance

Mycobacteria, especially Mycobacterium tuberculosis, are responsible for significant morbidity and mortality throughout the world.  Tuberculosis, spread by inhalation of airborne particles, is a serious public health threat.

It is important to be able to detect and identify mycobacteria in clinical specimens in a timely and accurate manner.  It has been demonstrated the recovery of mycobacteria is enhanced by use of automated broth culture systems such as the BACTEC MGIT 960 system.

Most mycobacteria grow at a relatively slow rate; therefore, the acid fast smear plays an important role in the early diagnosis of mycobacterial infections.  Also, the acid-fast stain serves to confirm the acid-fast nature of the recovered organisms and monitor patients on antimycobacterial therapy