We are proud to announce that with the introduction of a T- and B-Lymphocyte and Natural Killer Cell Profile tests, we started the establishing of a Centre of Excellence for Flow Cytometry, within our ICAD Laboratory in Abu Dhabi.

We are further working on expanding the test menu of the Center with more extensive immunophenotyping panels by flow cytometry, used to diagnose different leukemia and lymphoma types.

As the sample stability for this testing is extremely time sensitive, by establishing the Centre of Excellence for Flow Cytometry, we are aiming to meet the demand for this advanced testing in country, and reduce the dependence on sending samples abroad.  

 

Order Code

Order Code Name

CPT Code

Specimen type

Volume

Specimen container

Storage

Specimen stability

TAT*days

505370

T- and B-Lymphocyte and Natural Killer Cell Profile

86355, 86357, 86359, 86360

Whole blood

4 ml;

Min. volume: 1ml

Note: Sample should be submitted from Sunday to Thursday

 Cut-off time: Sunday to Wednesday - 8PM for Abu Dhabi, Dubai and Al Ain clients;

Thursday - 2PM for Abu Dhabi clients only

Lavender-top (EDTA) tube

Room temperature

Room temperature for 1 day

2 days

 

Flow Cytometric Analysis of Cell Surface Antigens (Immunophenotype)

Clinical Significance:

Peripheral blood lymphocytes are heterogeneous in nature. Based on biological function and cell-surface antigen expression, lymphocytes are classified into three major populations: T lymphocytes, B lymphocytes and NK (Natural Killer) lymphocytes.  There are various subsets of each of these individual populations with specific cell-surface markers and function. Each of these lymphocyte subpopulations has distinct effector and regulatory functions. The T cells (CD3+) are further divided into two broad categories, based on the expression and functional properties:  CD4+ helper/inducer and CD8+ suppressor/cytotoxic. B cells are identified by the expression of CD19+, while NK cells are typically identified by the coexpression of CD16+ and CD56+.

This flow-cytometric assay provides absolute (cells/mcL) and relative (%) quantitation for the main categories of T cells, B cells, and NK cells.  Abnormalities in the number and percent of T (CD3+), T-helper (CD4+), T-suppressor (CD8+), B (CD19+), and NK (CD16+, CD56+) lymphocytes have been described in a number of different disease conditions. In patients who are infected with HIV, the CD4+ count is measured for AIDS diagnosis and for initiation of antiviral therapy. The progressive loss of CD4 T-lymphocytes inpatients infected with HIV is associated with increased infections and complications.  It is also helpful as a preliminary screening assay for quantitative anomalies in any lymphocyte subset, whether related to malignancies or infection, especially for diagnosis and treatment.